p53 antibody

Catalog No. ABIN866051

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of p53.

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: Positive Control: A431. Cellular Localization: Cytoplasm, nucleus

Purification: Immunogen affinity purified

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml

Buffer: 1xTBS (pH7.4), 0.5% BSA, 25% Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53 Products)

Background: P53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. It acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. P53 is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. p53 is frequently mutated or inactivated in about 60% of cancers

Molecular Weight: ~70kDa

UniProt: Q91XH8

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect