SLC9A3 antibody

Catalog No. ABIN863199

Product Details anti-SLC9A3 Antibody

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC9A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level),Detects ~84 kDa.

Cross-Reactivity: Mouse, Rat

Purification: Protein A Purified

Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863199 was sufficient for detection of HNE3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-SLC9A3 antibody (ABIN863199)

Jones, Bailey, Murray, Lu, McNeilly, Schlötzer-Schrehardt, Lennon, Sado, Brownstein, Mullins, Kadler, Van Agtmael: "ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice." in: Disease models & mechanisms, Vol. 9, Issue 2, pp. 165-76, (2016) (PubMed).

Lee, Osis, Handlogten, Guo, Verlander, Weiner: "Effect of dietary protein restriction on renal ammonia metabolism." in: American journal of physiology. Renal physiology, Vol. 308, Issue 12, pp. F1463-73, (2015) (PubMed).

Bourgeois, Bounoure, Christensen, Ramakrishnan, Houillier, Devuyst, Wagner: "Haploinsufficiency of the ammonia transporter Rhcg predisposes to chronic acidosis: Rhcg is critical for apical and basolateral ammonia transport in the mouse collecting duct." in: The Journal of biological chemistry, Vol. 288, Issue 8, pp. 5518-29, (2013) (PubMed).

Target Details for SLC9A3

Target: SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))

Alternative Name: NHE3 (SLC9A3 Products)

Background: Sodium-hydrogen exchanger 3 (NHE3, Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).

Gene ID: 24784

NCBI Accession: NP_036786

UniProt: P26433

Pathways: Proton Transport