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CD25 antibody

IL2RA Reactivity: Human FACS Host: Mouse Monoclonal ITYV unconjugated

Catalog No. ABIN793425

Datasheet as PDF

Target See all CD25 (IL2RA) Antibodies

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
Reactivity
165

111

36

10

10

5

3

2

2

2

1

1
Human
Host
137

80

77

2

1

1
Mouse
Compatible Secondaries
Clonality
222

70

4
Monoclonal
Conjugate
117

33

28

25

22

11

6

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD25 antibody is un-conjugated
Application
218

86

54

53

44

44

23

18

15

13

8

8

6

6

4

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

Human IL2RA / CD25

Purification

Protein A/G purified

Immunogen

PHA-activated human T-lymphocytes.

Clone

ITYV

Isotype

IgG1

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IL2RA Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 1B5D12 unconjugated

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IL2RA Reactivity: Human WB, ELISA, IHC (fro), IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
IL2RA Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ELISA, IF/ICC Host: Mouse Monoclonal unconjugated

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IL2RA Reactivity: Human FACS Host: Mouse Monoclonal MEM-181 unconjugated

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IL2RA Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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IL2RA Reactivity: Human FACS Host: Mouse Monoclonal MEM-181 PerCP

anti-Interleukin 2 Receptor, alpha (IL2RA) antibody
IL2RA Reactivity: Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibody
IL2RA Reactivity: Human IHC, StM Host: Mouse Monoclonal IL2RA-2395 unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-185) antibody
IL2RA Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo

Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.08 % Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Aliquot to Avoid freeze/thaw cycles.

Storage

-20 °C

Storage Comment

Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Target

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name

IL2RA / CD25 (IL2RA Products)

Background

Name/Gene ID: IL2RA
Family: Interleukin

Synonyms: IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

Gene ID

3559

UniProt

P01589

Pathways

JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation