CD28 antibody (Extracellular Domain)
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- Target See all CD28 Antibodies
- CD28
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Binding Specificity
- AA 137-149, Extracellular Domain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This CD28 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Mouse CD28. The immunizing peptide represents a part of the extracellular domain.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Mouse (100%), Rat (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide KEKHLCHTQSSPK from an internal region of mouse CD28 (NP_031668.3). Percent identity with other species by BLAST analysis: Mouse (100%), Rat (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CD28 Primary Antibody
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-CD28 (CD28) antibodyCD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC
anti-CD28 (CD28) antibodyCD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) (AA 19-152) antibodyCD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibody (PE)CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE
anti-CD28 (CD28) (C-Term) antibodyCD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated
anti-CD28 (CD28) antibody (PerCP)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PerCP
anti-CD28 (CD28) antibody (APC)CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC
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- Application Notes
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Approved: Peptide-ELISA (1:16000), WB (0.3 - 1 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 40kD band observed in Mouse Thymus lysates (calculated MW of 25.2kD according to NP_031668.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1 μg/mL. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- CD28
- Alternative Name
- CD28 (CD28 Products)
- Background
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Name/Gene ID: CD28
Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule - Gene ID
- 940
- NCBI Accession
- NP_031668
- UniProt
- P10747
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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