VASP antibody (C-Term)

Catalog No. ABIN786360

Product Details anti-VASP Antibody

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SGRSGGGGLM EEMNAMLARR RKATQVGEKT PKDESANQEE PEARVPAQSE

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 79%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against VASP. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human VASP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 380 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP (VASP Products)

Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Alias Symbols: -
Protein Interaction Partner: ABI3, FGFR1OP, ABI2, SCGB2A2, CEP250, TP53, XIRP1, UBC, FAT1, FOXK1, PPP4R2, PAAF1, PRPF40A, RPP38, PRMT5, POP7, CUL4B, PSMA5, DDB1, APBB1, YWHAQ, NR4A1, YWHAE, DMRTB1, NPM1, VCAM1, SAT1, ITGA4, FN1, AMOT, SF3B2, SF3A3, ATP6V1F, CPNE3, SRSF9, UBE2N, UAP1,
Protein Size: 380

Molecular Weight: 42 kDa

Gene ID: 7408

NCBI Accession: NM_003370, NP_003361

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation