Albumin antibody

Catalog No. ABIN784420

Product Details anti-Albumin Antibody

Target: Albumin (ALB)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The defined antibody specificity is directed to Albumin as tested against Mouse sera. In Immunoelectrophoresis and double radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against Mouse serum and the purified Albumin. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in Double Radial Immunodiffusion with the following results: Bovine (-), Chicken (-), Canine (-), Goat (-), Guinea Pig (++), Hamster (+), Horse (+), Human (+), Monkey (+), Rat (++), Sheep (-), Swine (+). A negative cross-reaction in Double Radial Immunodiffusion does not exclude some reaction in more sensitive techniques.

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Highly purified Albumin isolated from Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 1 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin (ALB Products)

Background: Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin

Gene ID: 11657

NCBI Accession: NP_033784

Pathways: Lipid Metabolism