CD16 antibody

Catalog No. ABIN781725

Product Details anti-CD16 Antibody

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antibody recognizes CD16, the low affinity receptor for IgG (FcγRIII). It detects both isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity chromatography

Clone: C127

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute by adding 0.5 mL distilled water.

Buffer: Stock solution contains 0.4 mg/mL IgG, phosphate buffered saline pH 7.2 (PBS), 5 mg/mL bovine serum albumin (BSA), and 0.09 % sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized product can be stored at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for CD16

Target: CD16

Alternative Name: CD16 (CD16 Products)

Background: CD16 is expressed on the majority of NK cells, on granulocytes, monocytes and macrophages, and on a subset of T cells. CD16 is involved in antibody-dependent cellular cytotoxicity (ADCC). This molecule is encoded by FcγRIII-A or FcγRIII-B genes. The genetic heterogeneity of FcγRIII generates alternative membrane-anchored proteins with distinct signalling capacities when cross-linked by immune complexes. CD16 exists in two distinct forms, as a single-pass type I integral membrane protein (CD16a), and as a glycosyl phosphatidyl inositol (GPI) form (CD16b). In humans, the transmembrane form is expressed on NK cells and macrophages while the GPI linked form is expressed on granulocytes.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID: 2214

NCBI Accession: NP_000560

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process