Nestin antibody (AA 1295-1344)

Catalog No. ABIN6747184

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 1295-1344

Reactivity: Human, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NES / Nestin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1295-1344 of human NES (P48681, NP_006608). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey (92%), Marmoset, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Background: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gene ID: 10763

NCBI Accession: NP_006608

UniProt: P48681