OxLDL antibody

Catalog No. ABIN733148

Product Details anti-OxLDL Antibody

Target: OxLDL (Oxidized Low Density Lipoprotein (OxLDL))

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OxLDL antibody is un-conjugated

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Full length plasma protein (Human)

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-OxLDL antibody (ABIN733148)

Wang, Li, Zhen, Xu, Sun: "Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy." in: International journal of clinical and experimental pathology, Vol. 8, Issue 12, pp. 15696-707, (2016) (PubMed).

Martinez, Malireddi, Lu, Cunha, Pelletier, Gingras, Orchard, Guan, Tan, Peng, Kanneganti, Virgin, Green: "Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins." in: Nature cell biology, Vol. 17, Issue 7, pp. 893-906, (2015) (PubMed).

Wen, Liu, Liu, Harris, Yao, Ding, Nie, Lu, Chen, An, Zang, Teng: "OxLDL-targeted iron oxide nanoparticles for in vivo MRI detection of perivascular carotid collar induced atherosclerotic lesions in ApoE-deficient mice." in: Journal of lipid research, Vol. 53, Issue 5, pp. 829-38, (2012) (PubMed).

Target Details for OxLDL

Target: OxLDL (Oxidized Low Density Lipoprotein (OxLDL))

Alternative Name: ox-LDL (OxLDL Products)

Background:

Synonyms: ox-LDL: Cu2SO4 oxidized low density lipoprotein, oxidized low density lipoprotein, Low density lipoprotein, MDA oxidized LDL, MDA oxidized low density lipoprotein.

Background: Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.