CYP11A1 antibody (AA 321-420)

Catalog No. ABIN701530

Product Details anti-CYP11A1 Antibody

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Binding Specificity: AA 321-420

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP11A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CYP11A1 antibody (ABIN701530)

Sagaradze, Basalova, Kirpatovsky, Ohobotov, Grigorieva, Balabanyan, Kamalov, Efimenko: "Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential." in: Biomedicine & pharmacotherapy, Vol. 109, pp. 1428-1436, (2019) (PubMed).

Ommati, Heidari, Jamshidzadeh, Zamiri, Sun, Sabouri, Wang, Ahmadi, Javanmard, Seifi, Mousapour, Yeganeh: "Dual effects of sulfasalazine on rat sperm characteristics, spermatogenesis, and steroidogenesis in two experimental models." in: Toxicology letters, Vol. 284, pp. 46-55, (2018) (PubMed).

Medwid, Guan, Yang: "Prenatal exposure to bisphenol A disrupts adrenal steroidogenesis in adult mouse offspring." in: Environmental toxicology and pharmacology, Vol. 43, pp. 203-8, (2016) (PubMed).

Mahata, Zhang, Kolodziejczyk, Proserpio, Haim-Vilmovsky, Taylor, Hebenstreit, Dingler, Moignard, Göttgens, Arlt, McKenzie, Teichmann: "Single-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis." in: Cell reports, Vol. 7, Issue 4, pp. 1130-42, (2014) (PubMed).

Hu, Dong, Chen, Lu, Han, Qiu, Chen, Qin, Li, Gu, Xia, Sun, Li, Wang: "Low-dose monobutyl phthalate stimulates steroidogenesis through steroidogenic acute regulatory protein regulated by SF-1, GATA-4 and C/EBP-beta in mouse Leydig tumor cells." in: Reproductive biology and endocrinology : RB&E, Vol. 11, pp. 72, (2013) (PubMed).

Target Details for CYP11A1

Target: CYP11A1 (Cytochrome P450, Family 11, Subfamily A, Polypeptide 1 (CYP11A1))

Alternative Name: CYP11A1 (CYP11A1 Products)

Background:

Synonyms: CYP11A, CYPXIA1, P45SCC, Cholesterol side-chain cleavage enzyme, mitochondrial, Cholesterol desmolase, Cytochrome P45 11A1, Cytochrome P45(scc), CYP11A1

Background: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]

Gene ID: 1583

UniProt: P05108

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Cellular Response to Molecule of Bacterial Origin