VDAC1 antibody (AA 85-190)

Catalog No. ABIN686872

Product Details anti-VDAC1 Antibody

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Binding Specificity: AA 85-190

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VDAC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: May detect VDAC2 due to sequence similarity

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human VDAC1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-VDAC1 antibody (ABIN686872)

Wang, Lin, Zhang, Liu, Tan, Ma: "Penehyclidine hydrochloride prevents anoxia/reoxygenation injury and induces H9c2 cardiomyocyte apoptosis via a mitochondrial pathway." in: European journal of pharmacology, Vol. 797, pp. 115-123, (2017) (PubMed).

Hui, Lu, Han, Zhou, Liu, Li, Jin: "Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats." in: Acta histochemica, Vol. 119, Issue 4, pp. 392-399, (2017) (PubMed).

Tan, Chen, Ma: "Penehyclidine hydrochloride post-conditioning reduces ischemia/reperfusion-induced cardiomyocyte apoptosis in rats." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 4272-4278, (2017) (PubMed).

Xing, Yuan, Xu, Gu, Liang, Lu: "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model." in: Environmental toxicology and pharmacology, Vol. 40, Issue 2, pp. 592-9, (2015) (PubMed).

Manczak, Reddy: "Abnormal interaction of VDAC1 with amyloid beta and phosphorylated tau causes mitochondrial dysfunction in Alzheimer's disease." in: Human molecular genetics, Vol. 21, Issue 23, pp. 5131-46, (2012) (PubMed).

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: VDAC (VDAC1 Products)

Synonyms: vdac1 antibody, VDAC1 antibody, 5076 antibody, ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1 antibody, ATVDAC1 antibody, T22N4.9 antibody, T22N4_9 antibody, voltage dependent anion channel 1 antibody, PORIN antibody, VDAC-1 antibody, AL033343 antibody, Vdac5 antibody, fa13f11 antibody, wu:fa13f11 antibody, zgc:85830 antibody, voltage-dependent anion channel 1 S homeolog antibody, voltage-dependent anion channel 1 antibody, voltage dependent anion channel 1 antibody, vdac1.S antibody, vdac1 antibody, VDAC1 antibody, Vdac1 antibody

Background:

Synonyms: PORIN, VDAC-1, Voltage-dependent anion-selective channel protein 1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC1, VDAC

Background: Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules, in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

Gene ID: 7416

UniProt: P21796