Fascin antibody (pSer39)

Catalog No. ABIN673011

Product Details anti-Fascin Antibody

Target: Fascin (FSCN1)

Binding Specificity: pSer39

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Fascin antibody (ABIN673011)

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

Majchrzak, Lo Re, Gajewska, Bulkowska, Homa, Paw?owski, Motyl, Murphy, Król: "Migrastatin analogues inhibit canine mammary cancer cell migration and invasion." in: PLoS ONE, Vol. 8, Issue 10, pp. e76789, (2013) (PubMed).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: FSCN1 (FSCN1 Products)

Background:

Synonyms: HSN, SNL, p55, FAN1, Fascin, 55 kDa actin-bundling protein, Singed-like protein, FSCN1

Background: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.

Gene ID: 6624

UniProt: Q16658