OPN-A,b (AA 14-40), (N-Term) antibody

Catalog No. ABIN655514

Product Details

Target: OPN-A,b

Binding Specificity: AA 14-40, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the N-terminal region of human OPN-a/b.

Clone: RB19160

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:2000. WB: 1:2000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References

Tu, Li, Zeng, Deng, Gao, Xiao, Luo, Jiang, Li, Lei: "MicroRNA-127-5p regulates osteopontin expression and osteopontin-mediated proliferation of human chondrocytes." in: Scientific reports, Vol. 6, pp. 25032, (2018) (PubMed).

Wang, Wang, Zhang, Deng, Long: "NEAT1/miR-181c Regulates Osteopontin (OPN)-Mediated Synoviocyte Proliferation in Osteoarthritis." in: Journal of cellular biochemistry, Vol. 118, Issue 11, pp. 3775-3784, (2018) (PubMed).

Liang, Xu, Zhou, Liu, Ge, Chen, Tu: "MALAT1/miR-127-5p Regulates Osteopontin (OPN)-Mediated Proliferation of Human Chondrocytes Through PI3K/Akt Pathway." in: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 431-439, (2018) (PubMed).

Li, Jiang, Wang, Deng, Zeng, Tu, Li, Xiao, Gao, Luo, Lei: "Osteopontin Promotes Expression of Matrix Metalloproteinase 13 through NF-κB Signaling in Osteoarthritis." in: BioMed research international, Vol. 2016, pp. 6345656, (2016) (PubMed).

Target Details

Target: OPN-A,b

Background: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).

Molecular Weight: 35423

Gene ID: 6696

NCBI Accession: NP_000573, NP_001035147, NP_001035149, NP_001238758, NP_001238759

UniProt: P10451