SP1 antibody (C-Term)

Catalog No. ABIN655398

Product Details anti-SP1 Antibody

Target: SP1 (Sp1 Transcription Factor (SP1))

Binding Specificity: AA 677-707, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 677-707 amino acids from the C-terminal region of human SP1.

Clone: RB17350

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:500. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-SP1 antibody (ABIN655398)

Zhang, Wang, Qin, Liu, Sun, Liu, Kuang, Jiang, Zhang: "cis-Acting elements and trans-acting factors in the transcriptional regulation of raf kinase inhibitory protein expression." in: PLoS ONE, Vol. 8, Issue 12, pp. e83097, (2014) (PubMed).

Target Details for SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Alternative Name: SP1 (SP1 Products)

Background: Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Molecular Weight: 80693

Gene ID: 6667

NCBI Accession: NP_001238754, NP_003100, NP_612482

UniProt: P08047

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction