MED25 antibody (AA 112-140)

Catalog No. ABIN654162

Product Details anti-MED25 Antibody

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Binding Specificity: AA 112-140

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MED25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids of human MED25.

Clone: RB24225

Isotype: IgG

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-MED25 antibody (ABIN654162)

Han, Rha, Chi: "MED25 is a mediator component of HNF4α-driven transcription leading to insulin secretion in pancreatic beta-cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e44007, (2013) (PubMed).

Target Details for MED25

Target: MED25 (Mediator Complex Subunit 25 (MED25))

Alternative Name: MED25 (MED25 Products)

Background: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq].

Molecular Weight: 78171

Gene ID: 81857

NCBI Accession: NP_112235

UniProt: Q71SY5

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response