alpha Tubulin antibody
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- Target See all alpha Tubulin (TUBA1) Antibodies
- alpha Tubulin (TUBA1)
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Reactivity
- Saccharomyces cerevisiae
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Host
- Rat
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Conjugate
- This alpha Tubulin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)
- Specificity
- DS-MB-02478 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (2). The epitope recognised by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly- Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (5). This product is routinely tested in ELISA on Tubulin. Reacts with: Yeast, Mammals, Birds. Antibody reactivity and working conditions may vary between species.
- Characteristics
- Rat Anti-Tubulin alpha
- Purification
- Purified
- Immunogen
- Yeast tubulin
- Isotype
- IgG2a
- Top Product
- Discover our top product TUBA1 Primary Antibody
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- Restrictions
- For Research Use only
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- Concentration
- IgG concentration 1.0 mg/ml
- Buffer
- Phosphate buffered saline pH7.4
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- alpha Tubulin (TUBA1)
- Alternative Name
- Tubulin alpha (TUBA1 Products)
- Pathways
- Microtubule Dynamics
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