Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (TRITC)
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Rabbit
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Host
- Donkey
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Clonality
- Polyclonal
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Conjugate
- TRITC
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Cross-Reactivity
- Sheep (Ovine), Chicken, Goat, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
- Characteristics
- Donkey anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG and affinity purified using solid phase rabbit IgG.
- Purification
- Affinity purification
- Isotype
- IgG
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (Cy3)WB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
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- Application Notes
- Needs to be determined by end user for immunocytochemistry (ICC), immunohistochemistry ( IHC) and for flow cytometry.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- 4 °C
- Storage Comment
- Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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