Vinculin antibody (AA 873-922)

Catalog No. ABIN6746939

Product Details anti-Vinculin Antibody

Target: Vinculin (VCL)

Binding Specificity: AA 873-922

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Cow, Horse, Pig, Zebrafish (Danio rerio), Dog, Guinea Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human VCL / Vinculin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa873-922 of human VCL (P18206, NP_003364). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Seabass, Zebrafish (100%), Xenopus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-Vinculin antibody (ABIN6746939)

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, (1996) (PubMed).

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, (1994) (PubMed).

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: VCL / Vinculin (VCL Products)

Background: Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

Gene ID: 7414

NCBI Accession: NP_003364

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2