CYP11B1 antibody (AA 331-380)

Catalog No. ABIN6746765

Product Details anti-CYP11B1 Antibody

Target: CYP11B1 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 1 (CYP11B1))

Binding Specificity: AA 331-380

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP11B1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human CYP11B1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa331-380 of human CYP11B1 (P15538, NP_000488). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-CYP11B1 antibody (ABIN6746765)

Miyata, Kudo, Lee, Yang, Gelboin, Fernandez-Salguero, Kimura, Gonzalez: "Targeted disruption of the microsomal epoxide hydrolase gene. Microsomal epoxide hydrolase is required for the carcinogenic activity of 7,12-dimethylbenz[a]anthracene." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 23963-8, (1999) (PubMed).

Zhu, von Dippe, Xing, Levy: "Membrane topology and cell surface targeting of microsomal epoxide hydrolase. Evidence for multiple topological orientations." in: The Journal of biological chemistry, Vol. 274, Issue 39, pp. 27898-904, (1999) (PubMed).

Hassett, Aicher, Sidhu, Omiecinski: "Human microsomal epoxide hydrolase: genetic polymorphism and functional expression in vitro of amino acid variants." in: Human molecular genetics, Vol. 3, Issue 3, pp. 421-8, (1994) (PubMed).

Target Details for CYP11B1

Target: CYP11B1 (Cytochrome P450, Family 11, Subfamily B, Polypeptide 1 (CYP11B1))

Alternative Name: CYP11B1 (CYP11B1 Products)

Background: Name/Gene ID: CYP11B1
Family: Cytochrome P450

Synonyms: CYP11B1, CYPXIB1, Cytochrome P-450c11, Cytochrome P450C11, S11BH, Steroid 11-beta-hydroxylase, Steroid 11-beta-monooxygenase, CYP11B, Cytochrome p450 XIB1, FHI, p450C11

Gene ID: 1584

NCBI Accession: NP_000488

UniProt: P15538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis