PCNP antibody (C-Term)

Catalog No. ABIN6746506

Product Details anti-PCNP Antibody

Target: PCNP (PEST Proteolytic Signal Containing Nuclear Protein (PCNP))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Dog, Guinea Pig, Horse, Bat, Pig, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNP antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse PCNP

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of mouse Pcnp (Q6P8I4, NP_001019793). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Monkey, Xenopus (92%), Salmon, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PCNP antibody (ABIN6746506)

Bolton, Anthony, Perry: "Loss of the tight junction proteins occludin and zonula occludens-1 from cerebral vascular endothelium during neutrophil-induced blood-brain barrier breakdown in vivo." in: Neuroscience, Vol. 86, Issue 4, pp. 1245-57, (1998) (PubMed).

Fanning, Jameson, Jesaitis, Anderson: "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29745-53, (1998) (PubMed).

Ando-Akatsuka, Saitou, Hirase, Kishi, Sakakibara, Itoh, Yonemura, Furuse, Tsukita: "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 43-7, (1996) (PubMed).

Target Details for PCNP

Target: PCNP (PEST Proteolytic Signal Containing Nuclear Protein (PCNP))

Alternative Name: PCNP (PCNP Products)

Background: Name/Gene ID: PCNP

Synonyms: PCNP

Gene ID: 57092

NCBI Accession: NP_001019793

UniProt: Q8WW12