CORO2B antibody (AA 275-324)

Catalog No. ABIN6746343

Product Details anti-CORO2B Antibody

Target: CORO2B (Coronin, Actin Binding Protein, 2B (CORO2B))

Binding Specificity: AA 275-324

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Guinea Pig, Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CORO2B antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse CORO2B

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa275-324 of mouse Coro2b (NP_780693). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Panda, Dog, Turkey, Chicken (92%), Opossum, Zebra finch, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-CORO2B antibody (ABIN6746343)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Christmann, Kaina: "Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36256-62, (2000) (PubMed).

Palombo, Gallinari, Iaccarino, Lettieri, Hughes, DArrigo, Truong, Hsuan, Jiricny: "GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells." in: Science (New York, N.Y.), Vol. 268, Issue 5219, pp. 1912-4, (1995) (PubMed).

Target Details for CORO2B

Target: CORO2B (Coronin, Actin Binding Protein, 2B (CORO2B))

Alternative Name: CORO2B (CORO2B Products)

Background: Name/Gene ID: CORO2B

Synonyms: CORO2B, Coronin-like protein C, Clipin-C, Coronin-2B, Clipin C, CLIPINC, KIAA0925, Protein FC96

Gene ID: 10391

NCBI Accession: NP_780693

UniProt: Q9UQ03