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CD28 antibody

CD28 Reactivity: Human FACS, ELISA, Func Host: Mouse Monoclonal ANC28-1-5D10 unconjugated

Catalog No. ABIN610012

Datasheet as PDF

Target See all CD28 Antibodies

CD28
Reactivity
170

112

50

37

22

16

12

12

7

7

6

6

1

1

1

1
Human
Host
133

75

27

16

12

3

2
Mouse
Compatible Secondaries
Clonality
194

67

4
Monoclonal
Conjugate
98

32

28

20

13

5

4

4

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CD28 antibody is un-conjugated
Application
207

86

55

48

37

35

31

26

21

17

13

7

6

6

5

5

4

4

4

4

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), ELISA, Functional Studies (Func)

Specificity

CD28 expressed on T cells and also on BHK cells transfected with human CD28. This antibody has been characterized as a superagonist.

Purification

Protein A purified

Immunogen

Recombinant Human CD28 and HPB-MLT human tumor cells.

Type of Immunogen: Recombinant protein

Clone

ANC28-1-5D10

Isotype

IgG1 kappa

Top Product

Discover our top product CD28 Primary Antibody
anti-CD28 (CD28) (AA 1-120) antibody
CD28 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 FITC

anti-CD28 (CD28) antibody
CD28 Reactivity: Human, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC Host: Mouse Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) (AA 19-152) antibody
CD28 Reactivity: Human FACS, ELISA Host: Rabbit Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) antibody
CD28 Reactivity: Rat FACS, IP Host: Mouse Monoclonal JJ319 unconjugated

anti-CD28 (CD28) antibody (PE)
CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 PE

anti-CD28 (CD28) (C-Term) antibody
CD28 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-CD28 (CD28) antibody (PerCP)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 PerCP

anti-CD28 (CD28) antibody (APC)
CD28 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal CD28-2 APC

Application Notes

Approved: ELISA, Flo, Func

Usage: Function: ANC28.1 stimulates expression of IL-2 from CD28+ cells. Divalent ANC28.1 can induce Antigen Independent T cell proliferation. In combination with anti-CD81 mAb, it can prime naive T cells to acquire effector function independent of TCR ligation.

Not recommended for: WB

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate

Preservative

Gentamicin sulfate

Precaution of Use

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 4°C. Do not freeze.
Target

CD28

Alternative Name

CD28 (CD28 Products)

Background

Name/Gene ID: CD28

Synonyms: CD28, CD28 antigen, CD28 antigen (Tp44), Tp44, CD28 Molecule

Gene ID

940

UniProt

P10747

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response