CD19 antibody (AA 540-557)

Catalog No. ABIN609293

Product Details anti-CD19 Antibody

Target: CD19 (CD19 Molecule (CD19))

Binding Specificity: AA 540-557

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes the C-terminal cytoplasmic tail sequence (CGPDPAWGGGGRMGTWSTR) of human CD19, a 95kD cell surface glycoprotein, which is expressed by cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. Detects a band of ~95kD in Raji cell lysates under reducing conditions.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (94%) Elephant, Panda, Dog (89%) Pig (83%).

Purification: Protein G purified

Immunogen: CD19 peptide C-GPDPAWGGGGRMGTWSTR (C-terminus) coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (94%), Elephant, Panda, Dog (89%), Pig (83%).

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:100 - 1:200), IHC, IHC-P (1:100 - 1:200), IP, WB (1:100 - 1:200)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry (paraffin), Immunoprecipitation, and Flow Cytometry. Flow Cytometry: 1:100-1:200, 10 μL labels 10e6 cells in 100 μLImmunohistochemistry (paraffin): 1:100-1:200. Membrane permeabilization and antigen retrieval using heat treatment methods prior to staining of paraffin sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19 (CD19 Products)

Background: Name/Gene ID: CD19

Synonyms: CD19, B-lymphocyte antigen CD19, CD19 Molecule, Differentiation antigen CD19, T-cell surface antigen Leu-12, B4, CD19 antigen, CVID3

Gene ID: 930

UniProt: P15391

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway