CD166 antibody (AA 28-527) (Biotin)

Catalog No. ABIN602949

Product Details anti-CD166 Antibody (Biotin)

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Binding Specificity: AA 28-527

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD166 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, r  mMadCAM or rmOCAM.

Purification: Immunoaffinity purified

Immunogen: Purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM, aa 28-527, Accession No. Q13740).

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Sterile-filtered, lyophilized powder in PBS,  pH 7.2, BSA

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Target Details for CD166

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Alternative Name: ALCAM / CD166 (ALCAM Products)

Background: Name/Gene ID: ALCAM

Synonyms: ALCAM, CD166 antigen, MEMD, CD166

Gene ID: 214

UniProt: Q13740