E. Coli Elongation Factor TU antibody
Reactivity: Streptococcus, Bacillus, Deinococcus, E. coli, Pseudomonas, Pseudomonas cepacia (Burkholderia cepacia)
IF, ELISA, WB
Host: Mouse
Monoclonal
9E2
unconjugated
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- Target
- E. Coli Elongation Factor TU
- Reactivity
- Streptococcus, Bacillus, Deinococcus, E. coli, Pseudomonas, Pseudomonas cepacia (Burkholderia cepacia)
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunofluorescence (IF), ELISA, Western Blotting (WB)
- Specificity
- Recognizes Escherichia coli Elongation Factor TU at the very end of the N-terminus of the EF-Tu molecule (SKEKFE). Species cross-reactivity: Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, Deinococcus sp., and weakly cross-reacts with Mycobacterium tuberculosis.
- Purification
- Protein G purified
- Immunogen
- E. coli K-12 C600 Rif (pKT146) bacteria.
- Clone
- 9E2
- Isotype
- IgG2a
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- Application Notes
-
Approved: ELISA, IF, WB (1:50)
Usage: Suitable for use in ELISA, Immunofluorescence and Western Blot. Western Blot: 1:50 Bacterial EF-Tu reveals a molecular mass of 43-47kD, whereas archaeal EF-Tu reveals as either 80 or 60kD (ref 1). - Comment
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Target Species of Antibody: E. coli
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.1 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to Avoid repeated freezing and thawing.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
- Target
- E. Coli Elongation Factor TU
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