Histone 3 antibody (H3K4me3)

Catalog No. ABIN577703

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Brand: AbX™

Specificity: To confirm the specificity of the antisera a dot blot system was used with amounts of peptides from 10- 250 pmoles. Unmodified, monomethylated, dimethylated and trimethylated peptides surrounding the lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively. Lanes 5-8 contained peptides start- ing at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue at lysine 9 on Histone H3 that is unmodified, monomethylated, dimethylated and trimethylated respectively. Lanes 9-12 contained peptides encompassing the residue at lysine 27 on Histone H3 that is unmodi- fied, monomethylated, dimethylated and trimethylated respectively.

Cross-Reactivity (Details): Peptide immunogen sequence is highly conserved. Expected to react with Histone H3 from yeast to mam- mals.

Characteristics: Reacts specifically with modified trimethylated Lysine4 in Histone H3

Immunogen: Lysine4 trimethylated peptide of Histone H3

Application Details

Application Notes: Western blotting and ChIP. Other applications not yet tested.Suggested dilution, 1:100-1:500, ChIP: 30-50 μL AbX™ Anti TriMe Lys4 Histone H3 Rabbit pAb WesTeRN BLOTTINg
Acid treated HeLa (10 μg) extract was Antibody A007-100UL was used in ChIP with treated with a 1:100 dilution of antibody wild type yeast chromatin (lane 1) or A007-100UL prior to visualization. a strain with a point mutation in the code for Lysine 4 (lane 2).

Comment:

Reacts specifically with modified trimethylated Lysine4 in Histone H3 Supplied as a PBS solution Applications include Western blotting and ChIP

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate Buffered Saline at pH 7.2 containing 0.1 % Tween 20 and 0.09 % Kathon preservative

Preservative: Kathon CG

Storage: 4 °C

Storage Comment: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Synonyms: H-3 antibody, histocompatibility 3 antibody, H3 antibody

Background: The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Histones have been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. A diverse set of histone lysine methyltransferases has been identified, all but one of which contain a SET domain originally identified in the Drosophila, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation of these lysine residues induces the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules. The discovery in 2004 of the histone demethylase LSD1, followed by the Jumonji demethylases JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic process controlling cellular events.