Histone 3 antibody (H3K4me)

Catalog No. ABIN577701

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB)

Brand: AbX™

Specificity: To confirm the specificity of the antisera a dot blot system was used with amounts of peptdes from 10- 250 pmoles. Unmodified, monomethylated, dimethylated and trimethylated peptides surrounding the lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively. Lane 5 was also spotted with the monomethylated Lys4 peptide. Lanes 6-9 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue at lysine 9 on Histone H3 that is unmodified, monomethylated, dimethylated and trimethylated respectively.

Cross-Reactivity (Details): Peptide immunogen sequence is highly conserved. Expected to react with Histone H3 from yeast to mam- mals.

Characteristics: Reacts specifically with modified monomethylated Lysine4 in Histone H3

Purification: Purified

Immunogen: Lysine4 monomethylated peptide of Histone H3

Application Details

Application Notes: Western blotting. Other applications not yet tested.Suggested dilution, 1:500-1:2,000 AbX™ Anti MonoMe Lys4 Histone H3 Rabbit pAb WesteRN BLOttINg Acid treated HeLa extract was treated with a 1:500 dilution of antibody A005-100UL prior to vi- sualization.

Comment:

Reacts specifically with modified monomethylated Lysine4 in Histone H3 Supplied as a PBS solution Applications include Western blotting

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate Buffered Saline at pH 7.2 containing 0.01 % Tween 20 and 0.09 % Kathon preservative

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

Storage Comment: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Background: The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Histones have been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. A diverse set of histone lysine methyltransferases has been identified, all but one of which contain a SET domain originally identified in the Drosophila, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation of these lysine residues induces the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules. The discovery in 2004 of the histone demethylase LSD1, followed by the Jumonji demethylases JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic process controlling cellular events.