Histone 3 antibody (C-Term)

Catalog No. ABIN577700

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Brand: AbX™

Cross-Reactivity (Details): Peptide immunogen sequence is highly conserved. Expected to react with Histone H3 from yeast to mammals.

Characteristics: Reacts with modified Histone H3

Purification: Purified

Immunogen: C-Terminal peptide of Histone H3 which should be non-modified.

Application Details

Application Notes: Western blotting and ImmunoprecipitationSuggested dilution, 1:500-1:1,500 ChIP: 50 μL AbX™ Anti Histone H3 Rabbit pAb WesteRN BlOttINg 5 μL of acid treated HeLa extracts or recombinant Histone H3 were treated with a 1:500 dilution of antibody A004-100UL prior to visualization. Lane 1: HeLa Extract Lane 2: HeLa cells treated with sodium butyrate Lane 3: Recombinant human Histone H3 (0.2 μg)

Comment:

Reacts with modified Histone H3 Supplied as a PBS solution Applications include Western blotting and ChIP

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate Buffered Saline at pH 7.2 containing 0.1 % Tween 20 and 0.09 % Kathon preservative

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C

Storage Comment: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Background: Chromosomal surfaces are ornamented with a variety of posttranslational modifications of histones, which are required for the regulation of many of the DNA-templated processes. Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminus tail, H3 is involved with the 'beads on a string' structure of the nucleosome. Histone H3 is the most extensively post-translationally modified of the five histones. The term "Histone H3" alone is purposely ambigu- ous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the regulation of genes. For example, histone methylation either on their arginine and/ or lysine residues, yields a more diverse array of cellular functions.