ELISA. Immunohistochemistry on Paraffin Sections: Use Ab at 5-10 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Ovariancarcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer, before the addition of Glycerol to 40 %.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta coded by two different genes. They possess five immunoglobulin like domains that are involved in ligand binding. Two tyrosine kinase domains are separated by a kinase insert to which PI-3 kinase can bind. PDGF AA can bind autophosphorylate and activate only PDGFR, alpha while PDGF BB can bind and activate to both PDGFR, alpha and beta.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha