RIPK3 antibody (Middle Region)

Catalog No. ABIN504563

Product Details anti-RIPK3 Antibody

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: ETPGLEGLKE LMQLCWSSEP KDRPSFQECL PKTDEVFQMV ENNMNAAVST

Predicted Reactivity: Dog: 92%, Horse: 92%, Human: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against RIPK3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RIPK3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 518 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RIPK3 antibody (ABIN504563)

Duman, Alcigir, Yavuz: "Necroptosis mediated by receptor interacting protein kinase 3 as critical players in experimental congenital hypothyroidism related neuronal damage." in: Northern clinics of Istanbul, Vol. 8, Issue 5, pp. 472-478, (2021) (PubMed).

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: RIPK3 (RIPK3 Products)

Background: RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: RIP3
Protein Interaction Partner: BMH1, RFX6, FADD, RIPK1, CASP8, INPP5K, CDC37, STIP1, PYGL, FKBP5, FKBP4, CTNND1, UBC, XIAP, BIRC3, BIRC2, TICAM1, TRAF2, TNFRSF1A,
Protein Size: 518

Molecular Weight: 57 kDa

Gene ID: 11035

NCBI Accession: NM_006871, NP_006862

UniProt: Q9Y572

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades