RPA1 antibody (Middle Region)

Catalog No. ABIN503995

Product Details anti-RPA1 Antibody

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig, Cow, Dog, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TLWGEDADKF DGSRQPVLAI KGARVSDFGG RSLSVLSSST IIANPDIPEA

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against RPA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 616 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RPA1

Target: RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name: RPA1 (RPA1 Products)

Background: RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. RPA1 binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing
Alias Symbols: HSSB, REPA1, RF-A, RP-A, RPA70, MST075
Protein Interaction Partner: STRA13, UBC, MTUS2, APITD1, PCNA, SUMO2, FBXO18, ATRX, MAP4, CAPRIN1, LMO7, LIG3, LDHA, ITGB1, IMPDH2, ILF3, ILF2, HSPB1, HSPA8, HSPA5, HNRNPU, HNRNPL, HNRNPK, HNRNPH1, HNRNPF, HNRNPD, HNRNPAB, HNRNPA2B1, HNRNPA1, HCFC1, GTF3C2, GTF2I, GTF2H3, GAPDH, XRCC
Protein Size: 616

Molecular Weight: 68 kDa

Gene ID: 6117

NCBI Accession: NM_002945, NP_002936

UniProt: P27694

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA