FBXO33 antibody (Middle Region)

Catalog No. ABIN503806

Product Details anti-FBXO33 Antibody

Target: FBXO33 (F-Box Protein 33 (FBXO33))

Binding Specificity: Middle Region

Reactivity: Rat, Human, Mouse, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO33 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VIDTSGFPDL SDNRNEDPLV LLAWRCTKLS LLAIHGYTVW AHNLIAIARL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against FBXO33. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXO33

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 555 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FBXO33

Target: FBXO33 (F-Box Protein 33 (FBXO33))

Alternative Name: FBXO33 (FBXO33 Products)

Background: FBXO33 is the substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO33 probably recognizes and binds to phosphorylated target proteins. Recognizes YBX1.Members of the F-box protein family, such as FBXO33, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].
Alias Symbols: Fbx33, c14_5247, BMND12
Protein Interaction Partner: CUL1, YBX1, UBC,
Protein Size: 555

Molecular Weight: 62 kDa

Gene ID: 254170

NCBI Accession: NM_203301, NP_976046

UniProt: Q7Z6M2