NUMA1 antibody (C-Term)

Catalog No. ABIN503163

Product Details anti-NUMA1 Antibody

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Dog, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AQRLQAQLNE LQAQLSQKEQ AAEHYKLQME KAKTHYDAKK QQNQELQEQL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NUMA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NUMA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 2115 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NUMA1 antibody (ABIN503163)

Kilpivaara, Rantanen, Tamminen, Aittomäki, Blomqvist, Nevanlinna: "Comprehensive analysis of NuMA variation in breast cancer." in: BMC cancer, Vol. 8, pp. 71, (2008) (PubMed).

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NUMA1 (NUMA1 Products)

Background: NuMA is a structural element in maintaining nuclear integrity. A critical spindle pole-associated mechanism, called the END (Emi1/NuMA/dynein-dynactin) network, spatially restricts APC/C activity in early mitosis. The entire coding region and exon-intron boundaries of NuMa were screened in 92 familial breast cancer patients. But the results do not support the role of NuMA variants as breast cancer susceptibility alleles.
Alias Symbols: NUMA
Protein Interaction Partner: CCDC57, GMCL1, BRAP, DVL3, DVL2, DVL1, UBC, LGR4, ERG, RPA3, RPA2, RPA1, RNF2, EZH2, BMI1, SUZ12, EED, YWHAQ, LMNA, CBX6, WHSC1, HSP90AB1, HSP90AA1, ESR1, CDK6, VCP, SMURF1, MDC1, TP53BP1, ECT2, NCSTN, DDB1, EMILIN1, DYNLL1, RAE1, NUP98, CDK2, TERF2IP, TE
Protein Size: 2115

Molecular Weight: 238 kDa

Gene ID: 4926

NCBI Accession: NM_006185, NP_006176

UniProt: Q14980

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase