HS2ST1 antibody (Middle Region)

Catalog No. ABIN502980

Product Details anti-HS2ST1 Antibody

Target: HS2ST1 (Heparan Sulfate 2-O-Sulfotransferase 1 (HS2ST1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Zebrafish (Danio rerio), Cow, Dog, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HS2ST1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GVTEELEDFI MLLEAALPRF FRGATELYRT GKKSHLRKTT EKKLPTKQTI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against HS2ST1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HS2ST1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 356 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HS2ST1

Target: HS2ST1 (Heparan Sulfate 2-O-Sulfotransferase 1 (HS2ST1))

Alternative Name: HS2ST1 (HS2ST1 Products)

Background: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. HS2ST1 is a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes heparan sulfate 2-O-sulfotransferase, a member of the heparan sulfate biosynthetic enzyme family. This family member transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation.
Alias Symbols: FLJ11317, KIAA0448, MGC131986, dJ604K5.2
Protein Size: 356

Molecular Weight: 42 kDa

Gene ID: 9653

NCBI Accession: NM_012262, NP_036394

UniProt: Q7LGA3

Pathways: Glycosaminoglycan Metabolic Process, Tube Formation, SARS-CoV-2 Protein Interactome