NR5A1 antibody (Middle Region)

Catalog No. ABIN502654

Product Details anti-NR5A1 Antibody

Target: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Guinea Pig, Dog, Horse, Rabbit, Cow, Sheep, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR5A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: SLHGPEPKGL AAGPPAGPLG DFGAPALPMA VPGAHGPLAG YLYPAFPGRA

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 91%, Horse: 86%, Human: 100%, Pig: 92%, Rabbit: 79%, Rat: 86%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against NR5A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NR5A1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 461 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NR5A1 antibody (ABIN502654)

Derebecka-Holysz, Lehmann, Holysz, Trzeciak: "SMAD3 inhibits SF-1-dependent activation of the CYP17 promoter in H295R cells." in: Molecular and cellular biochemistry, Vol. 307, Issue 1-2, pp. 65-71, (2007) (PubMed).

Target Details for NR5A1

Target: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Alternative Name: NR5A1 (NR5A1 Products)

Background: NR5A1 is an important regulator of steroidogeneisis which is present in human skin and its appendages. It plays a role in regulating p450scc expression with TReP-132 and CBP/p300. The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: AD4BP, ELP, FTZ1, FTZF1, SF-1, SF1, POF7, SPGF8, SRXY3
Protein Interaction Partner: NCK2, NR0B1, RAD54L2, THOC2, ALYREF, EIF4A3, DDX39B, UBC, BARD1, ELAVL1, TCF7L2, CTNNB1, CREBBP, CXXC1, PNRC2, SOX8, PIAS1, ZNF653, TRERF1, EDF1, GATA4, GTF2B, SOX2, SOX9, TBX19, NCOA1, NRIP1, PITX1, SMARCD3, PROX1, AR, SP1, NFYA, MAPK1, JUN, NFKB2,
Protein Size: 461

Molecular Weight: 52 kDa

Gene ID: 2516

NCBI Accession: NM_004959, NP_004950

UniProt: Q13285

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Maintenance of Protein Location