SRD5A2 antibody (N-Term)

Catalog No. ABIN502491

Product Details anti-SRD5A2 Antibody

Target: SRD5A2 (Steroid-5-alpha-Reductase, alpha Polypeptide 2 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 2) (SRD5A2))

Binding Specificity: N-Term

Reactivity: Human, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRD5A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MQVQCQQSPV LAGSATLVAL GALALYVAKP SGYGKHTESL KPAATRLPAR

Predicted Reactivity: Human: 100%, Pig: 86%, Rabbit: 91%

Characteristics: This is a rabbit polyclonal antibody against SRD5A2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SRD5A2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 254 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SRD5A2 antibody (ABIN502491)

Bertin, Dury, Ouellet, Pelletier, Labrie: "Localization of the androgen-synthesizing enzymes, androgen receptor, and sex steroids in the vagina: possible implications for the treatment of postmenopausal sexual dysfunction." in: The journal of sexual medicine, Vol. 11, Issue 8, pp. 1949-61, (2014) (PubMed).

Target Details for SRD5A2

Target: SRD5A2 (Steroid-5-alpha-Reductase, alpha Polypeptide 2 (3-Oxo-5 alpha-Steroid delta 4-Dehydrogenase alpha 2) (SRD5A2))

Alternative Name: SRD5A2 (SRD5A2 Products)

Background: This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result
Alias Symbols: MGC138457
Protein Size: 254

Molecular Weight: 28 kDa

Gene ID: 6716

NCBI Accession: NM_000348, NP_000339

UniProt: Q28892

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis