Cyclin B1 antibody (C-Term)

Catalog No. ABIN502406

Product Details anti-Cyclin B1 Antibody

Target: Cyclin B1 (CCNB1)

Binding Specificity: C-Term

Reactivity: Human, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AKNVVMVNQG LTKHMTVKNK YATSKHAKIS TLPQLNSALV QDLAKAVAKV

Predicted Reactivity: Cow: 95%, Dog: 95%, Guinea Pig: 85%, Horse: 90%, Human: 100%, Pig: 95%, Rabbit: 95%, Rat: 80%

Characteristics: This is a rabbit polyclonal antibody against CCNB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CCNB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 433 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1 (CCNB1 Products)

Background: CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CCNB
Protein Interaction Partner: CDC27, CDC20, CDH1, ANAPC11, FZR1, ANAPC4, SIRT1, BTRC, CDC16, UBC, CDKN1A, VCP, Samhd1, CKS2, CKS1B, RHOBTB3, CDK1, CDK5, CDK2, SQSTM1, PTMA, TP73, TP53BP1, POLA1, RB1, CDK7, HIST1H1B, CDC6, CCNB1IP1, MFN1, MARCH5, PRC1, HIST1H1A, SDHAF2, NDC80, CDT1, RP
Protein Size: 433

Molecular Weight: 48 kDa

Gene ID: 891

NCBI Accession: NM_031966, NP_114172

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase