STAT5A antibody (C-Term)

Catalog No. ABIN501656

Product Details anti-STAT5A Antibody

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Sheep, Pig, Dog, Horse, Rabbit, Goat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT5A antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DQAPSPAVCP QAPYNMYPQN PDHVLDQDGE FDLDETMDVA RHVEELLRRP

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 92%, Pig: 93%, Rabbit: 86%, Rat: 92%, Sheep: 93%

Characteristics: This is a rabbit polyclonal antibody against STAT5A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human STAT5A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 794 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-STAT5A antibody (ABIN501656)

Murayama, Ohmori, Fujimura, Minami, Yasuzawa-Tanaka, Kuroda, Oie, Daitoku, Okuwaki, Nagata, Fukamizu, Kimura, Shimizu, Yanagisawa: "Epigenetic control of rDNA loci in response to intracellular energy status." in: Cell, Vol. 133, Issue 4, pp. 627-39, (2008) (PubMed).

Target Details for STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Alternative Name: STAT5A (STAT5A Products)

Background: STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGF, STAT5
Protein Interaction Partner: TCF12, NLK, LEF1, NARF, CBL, UBC, ESR1, HELZ, UGT2B4, STAT5A, POM121C, WASH2P, AQP11, ZNF596, MRPS6, TXNDC11, RBMX, INSR, GLUD1, FN1, EPAS1, CYP2C9, CSNK2B, JAK2, BTK, EPOR, CRKL, SOCS7, NFKB1, ERBB4, EP300, MYB, SQSTM1, GNB2, SMARCA4, PGR, FGFR2, JAK3, C
Protein Size: 794

Molecular Weight: 91 kDa

Gene ID: 6776

NCBI Accession: NM_003152, NP_003143

UniProt: P42229

Pathways: JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation