SART3 antibody (Middle Region)

Catalog No. ABIN501268

Product Details anti-SART3 Antibody

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Dog, Horse, Cow, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SART3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VKDLRLVTNR AGKPKGLAYV EYENESQASQ AVMKMDGMTI KENIIKVAIS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 91%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against SART3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SART3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 963 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SART3 antibody (ABIN501268)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for SART3

Target: SART3 (Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))

Alternative Name: SART3 (SART3 Products)

Background: SART3 is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. SART3 is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This protein is thought to be involved in the regulation of mRNA splicing. The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: KIAA0156, MGC138188, RP11-13G14, TIP110, p110(nrb), P100, p110, DSAP1
Protein Interaction Partner: APBB1, PRPF3, Usp15, AMOT, SF3B1, NUP160, LRPPRC, THRAP3, BCLAF1, VARS, UBC, SUMO2, RAB1A, NEFM, IARS, HIF1A, DSG2, DHX9, MEPCE, HIST2H2BE, HIST2H2AC, RNF2, BMI1, RUFY1, NRBP1, PSME4, AKAP2, COPS5, NUP50, POP7, ARHGEF10, PSMB3, PSMA5, PSMA3, POLR2H, CAST,
Protein Size: 963

Molecular Weight: 110 kDa

Gene ID: 9733

NCBI Accession: NM_014706, NP_055521

UniProt: Q15020

Pathways: Ribonucleoprotein Complex Subunit Organization