NLRX1 antibody (Internal Region)

Catalog No. ABIN499126

Product Details anti-NLRX1 Antibody

Target: NLRX1 (NLR Family Member X1 (NLRX1))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRX1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-NFSGETLDST DPSN

Specificity: This antibody is expected to recognize both reported isoforms (NP_078894.2 and NP_733840.1).

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to Human NLRX1 (NP_078894.2 and NP_733840.1). Genename: NLRX1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL. Detects a band of approx. 100 kDa in Human Breast cancer lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NLRX1

Target: NLRX1 (NLR Family Member X1 (NLRX1))

Alternative Name: NLRX1 / NOD9 (NLRX1 Products)

Background: Member of the NLRP family. Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction. Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen speciesSynonyms: Caterpiller 11.3, NLR family member X1, NOD26, NOD5, Nucleotide-binding oligomerization domain protein 26, Nucleotide-binding oligomerization domain protein 5, Nucleotide-binding oligomerization domain protein 9

Molecular Weight: 108kDa (NP_078894.2) and 102kDa (NP_733840.1).

Gene ID: 79671

NCBI Accession: NP_078894

UniProt: Q86UT6

Pathways: SARS-CoV-2 Protein Interactome