IGF2BP1 antibody (Internal Region)

Catalog No. ABIN498076

Product Details anti-IGF2BP1 Antibody

Target: IGF2BP1 (Insulin-Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This IGF2BP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-EKVFAEHKIS YSGQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_006537.3, NP_001153895.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the Internal region of the protein sequence according to NP_006537.3, NP_001153895.1. Genename: IGF2BP1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 65 kDa in lysates of cell line K562.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IGF2BP1

Target: IGF2BP1 (Insulin-Like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1))

Alternative Name: IGF2BP1 (IGF2BP1 Products)

Background: IGF2BP1 is a memember of the insulin like growth factor 2 mRNA binding protein family. IGF2BP1 contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin like growth factor 2, beta actin and beta transducin repeat containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found.Synonyms: CRD-BP, CRDBP, Coding region determinant-binding protein, IGF-II mRNA-binding protein 1, Insulin-like growth factor 2 mRNA-binding protein 1, VICKZ family member 1, VICKZ1, ZBP-1, ZBP1, Zip code-binding protein 1, Zipcode-binding protein 1

Molecular Weight: 63.5kDa according to NP_006537.3

Gene ID: 10642

NCBI Accession: NP_006537

UniProt: Q9NZI8