POU6F2 antibody (N-Term)

Catalog No. ABIN498070

Product Details anti-POU6F2 Antibody

Target: POU6F2 (POU Class 6 Homeobox 2 (POU6F2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This POU6F2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: RSEMNAELRG EDK-C

Specificity: This antibody is expected to recognize both reported isoforms (NP_009183.3, NP_001159490.1).

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Bovine.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with from the N Terminus of the protein sequence according to NP_009183.3, NP_001159490.1. Genename: POU6F2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/16000. Western blot: 0.3-1 μg/mL. Detects a band of Approx 65 kDa in Mouse Eye lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POU6F2

Target: POU6F2 (POU Class 6 Homeobox 2 (POU6F2))

Alternative Name: POU6F2 (POU6F2 Products)

Background: POU6F2 (POU class 6 homeobox 2) is a member of the POU domain family of transcription factors. It recognizes and binds to the DNA sequence 5'-ATGCAAAT-3' and may be involved in the early steps in the differentiation of amacrine and ganglion cells.Synonyms: POU domain class 6 transcription factor 2, RPF-1, RPF1, Retina-derived POU domain factor 1

Molecular Weight: Human and Mouse: 73.3 kDa (Human NP_009183.3 and Mouse NP_778171.2)

Gene ID: 11281

NCBI Accession: NP_009183

UniProt: P78424