POU2F3 antibody (Internal Region)
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- Target See all POU2F3 Antibodies
- POU2F3 (POU Class 2 Homeobox 3 (POU2F3))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Rat, Dog, Cow
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This POU2F3 antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Sequence
- C-EPHLEASQHL PVPKH
- Specificity
- Recognizes POU2F3.
- Cross-Reactivity (Details)
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Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Immunogen
- Peptide with sequence from the internal region of the protein sequence according to NP_055167.2. Genename: POU2F3
- Top Product
- Discover our top product POU2F3 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell lines HEK293 andHepG2 (calculated MW of 47.0 kDa according to NP_055167.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- POU2F3 (POU Class 2 Homeobox 3 (POU2F3))
- Alternative Name
- POU2F3 (POU2F3 Products)
- Background
- POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes.Synonyms: OTF-11, OTF11, Oct-11, Octamer-binding protein 11, Octamer-binding transcription factor 11, PLA-1, PLA1, POU domain class 2 transcription factor 3, Skn-1
- Gene ID
- 25833
- NCBI Accession
- NP_055167
- UniProt
- Q9UKI9
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