SEC23A antibody (Internal Region)

Catalog No. ABIN488037

Product Details anti-SEC23A Antibody

Target: SEC23A (Sec23 Homolog A (SEC23A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse

Host: Goat

Clonality: Polyclonal

Conjugate: This SEC23A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Recognizes SEC23A.

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat, Equine (Horse), Canine (Dog) and Bovine.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ANRAATTGHVID, from the internal region of the protein sequence according to NP_006355.2. Genename: SEC23A

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 100 kDa band observed in lysates of NIH3T3 (calculatedMW of 86.0 kDa according to Human NP_006355.2 and to Mouse NP_033173.2 ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SEC23A

Target: SEC23A (Sec23 Homolog A (SEC23A))

Alternative Name: SEC23A (SEC23A Products)

Background: The regulation of intracellular vesicular trafficking is mediated by specific families of proteins that are involved in vesicular budding, translocation, and fusion with target membranes. The protein trafficking between endoplasmic reticulum (ER) and Golgi apparatus is mediated by two distinct membrane coat complexes, COPI and COPII. Two Coat protein II (COPII) proteins are necessary to generate secretory vesicles at the endoplasmic reticulum. COPI consist of 7 protein subunits includinga, b, b1, d, e, d, z. COPI is also responsible for the retrograde golgi to ER transport f dilysine-tagged proteins. Sec23A is a functional human counter part of the yeast COPII component Sec23p which suggests that it plays similar role in the mammalian protein export from ER. Both Sec 23 isoforms (Sec23A and Sec23B) have similar molecular weight 85 kDa and are co-expressed in many human tissues at varying levels of expression. In yeast, the Sec24p protein is the only COPII component in which two close orthologues have been identified. Sec-23p is a component of coat protein II (COPII) coated vesicles involved in protein export from the endoplasmic reticulum (Mizoguchi et. al., 2000). The COPII is required for the vesicle budding from the endoplasmic reticulum and is consist of two heterodimeric subcomplexes, Sec23p/Sec24p and Sec13p/Sec31p and a small GTPase Ser1p (Higashio et al., 2000). Recent data also suggest that Sec23p/Sec24p complexes are in addition to its role in the shaping up of the vesicle, the Sec23-24p complex may be implicated in cargo selection and trafficking of the amount of cargo with in the cells.Synonyms: Protein transport protein Sec23A, SEC23-related protein A

Gene ID: 10484

NCBI Accession: NP_006355

UniProt: Q15436