KCNN2 antibody (Internal Region)

Catalog No. ABIN487968

Product Details anti-KCNN2 Antibody

Target: KCNN2 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 2 (KCNN2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This KCNN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-ESYDKHVTYN AER

Specificity: This antibody is expected to recognize both isoforms of KCNN2 (NP_067627.2, NP_740721.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_067627.2, NP_740721.1. Genename: KCNN2

Application Details

Application Notes: Peptide ELISA: 1/64000. Western blot: Approx 70 kDa and 20 kDa bands observed in Human Liver lysates (calculatedMW of 63.0 kDa according to NP_067627.2 and of 26.0 kDa according to NP_740721.1). Recommended concentration: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for KCNN2

Target: KCNN2 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 2 (KCNN2))

Alternative Name: KCNN2 (KCNN2 Products)

Background: A widely expressed protein forming a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.Synonyms: KCNN2, SK2, Small conductance calcium-activated potassium channel protein 2

Gene ID: 3781

NCBI Accession: NP_067627

UniProt: Q9H2S1