Syndecan 1 antibody

Catalog No. ABIN487501

Product Details anti-Syndecan 1 Antibody

Target: Syndecan 1 (SDC1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Syndecan 1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes CD138/Syndecan-1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: SYND1, SDC1, SDC

Purification: Protein-A Sepharose Chromatography of hybridoma supernatant.

Immunogen: XG1 and U266 myeloma cell lines. Remarks: Hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/cmouse splenocyte.

Clone: 15-11-14

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: KM3, RPMI8226, U226. Immunohistochemistry on Paraffin Sections: 1-5 μg/mL. Heat treatment is necessary for paraffin embedded sections. Microwave oven: 2 times for 10 minutes each in 10 mM citrate buffer ( pH 6.5). Positive Controls: Lung, Adenocarcinoma, Tonsil. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow cytometric analysis for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet aftertapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 40 µl of the primary antibody at the concentration of as suggest in theAPPLICATIONS diluted in the washing buffer. Mix well and incubate for 30 minutes at roomtemperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 7) Add 30 µL of 1: 40 FITC conjugated anti-mouse IgG diluted with the washing buffer. Mixwell and incubate for 15 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive controls for Flow cytometry: KM3, RPMI8226, U226Flow cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all step described below. 1) Add 20 µL of the anti-Syndecan-1/CD138 monoclonal antibody (50 µg/mL) diluted withwashing buffer [PBS containing 2% fetal calf serum (FCS) and 0. 1% NaN3] into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Add 2 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 4) Add 50 µL of secondary antibody 1: 40 FITC conjugated anti-Mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature. 5) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Immunohistochemical Staining for Paraffin-Embedded Sections: SAB method1) Deparaffinize the sections with Xylene 3 times for 3-5 minutes each.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: CD138 / Syndecan-1 (SDC1 Products)

Background: Syndecan-1 (CD138) is a heparan sulfate proteoglycan expressed on the surface of plasma cel1s and some epithelial cells where it mediates cell-cell and cell-extracellular matrix adhesion, and may facilitate the binding of growth factors to their high-affinity receptors as shown for basic fibroblast growth factor (b-FGF). It has been reported that altered expression of syndecan-1 in several tumors such as colorectal cancer, esophageal carcinoma, lung carcinoma, gastric cancer and multiple myeloma, and alteration of syndecan-1 expression in the tumors correlated with patients survival rate, stage, or metastasis.Synonyms: SDC, SDC1, SYND1

Gene ID: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism