ATP6V0A1 antibody (N-Term)

Catalog No. ABIN487206

Product Details anti-ATP6V0A1 Antibody

Target: ATP6V0A1 (ATPase, H+ Transporting, Lysosomal V0 Subunit A1 (ATP6V0A1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Dog, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0A1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RDLNPDVNVF QRKFVNEVRR CEEMDRKLRF VEKEIRKANI PIMDTGENPE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ATP6V0A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP6V0A1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 831 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATP6V0A1 antibody (ABIN487206)

Leiva-Rodríguez, Romeo-Guitart, Marmolejo-Martínez-Artesero, Herrando-Grabulosa, Bosch, Forés, Casas: "ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons." in: Cell death & disease, Vol. 9, Issue 6, pp. 626, (2019) (PubMed).

Antonacopoulou, Grivas, Skarlas, Kalofonos, Scopa, Kalofonos: "POLR2F, ATP6V0A1 and PRNP expression in colorectal cancer: new molecules with prognostic significance?" in: Anticancer research, Vol. 28, Issue 2B, pp. 1221-7, (2008) (PubMed).

Target Details for ATP6V0A1

Target: ATP6V0A1 (ATPase, H+ Transporting, Lysosomal V0 Subunit A1 (ATP6V0A1))

Alternative Name: ATP6V0A1 (ATP6V0A1 Products)

Background: ATP6V0A1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. ATP6V0A1 is one of three A subunit proteins and it is associated with clathrin-coated vesicles. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. The occurrence of splice variants encoding different protein products has been reported, but the full-length natures of these transcripts have not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ATP6N1, ATP6N1A, DKFZp781J1951, Stv1, VPP1, Vph1, a1
Protein Interaction Partner: SEH1L, ZNF267, SNX4, UBC, CLIC1, MARCKSL1, MRPL44, SLC30A5, MRPL50, PTRH2, ATP6V1D, MRPL46, SCFD1, VAMP2, SRSF3, RPS18, RPS15, SLC25A3, PA2G4, NONO, ATP6V1E1, ATP6V1B1,
Protein Size: 831

Molecular Weight: 96 kDa

Gene ID: 535

NCBI Accession: NM_005177, NP_005168

UniProt: Q93050

Pathways: Transition Metal Ion Homeostasis, Proton Transport