UBTF antibody (Middle Region)

Catalog No. ABIN487082

Product Details anti-UBTF Antibody

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Cow, Rabbit, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBTF antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LESLPEEEQQ RVLGEEKMLN INKKQATSPA SKKPAQEGGK GGSEKPKRPV

Predicted Reactivity: Cow: 86%, Guinea Pig: 79%, Horse: 77%, Human: 100%, Mouse: 93%, Pig: 86%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against UBTF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBTF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 764 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBTF

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Alternative Name: UBTF (UBTF Products)

Background: UBTF is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1. Two UBTF polypeptides, of 94 and 97 kD, exist in the human. UBTF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxesUpstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: NOR-90, UBF, UBF1, UBF-1
Protein Interaction Partner: UBC, MDM2, RNF2, Dlg4, ESR1, CDK4, CDK2, CCNE1, CCND1, WDR70, YEATS4, RB1, GRK5, KAT5, SIRT7, POLR1A, MYBBP1A, HDAC1, RUNX2, SUMO2, SMARCA5, MAPK3, MAPK1, SUMO1, PIK3CA, IRS1, TAF1D, tat, MCRS1, TLE1, ACTN4, POLR1E, CHGB, SMURF1, TCOF1, MAD1L1, ZMYM2, SER
Protein Size: 764

Molecular Weight: 89 kDa

Gene ID: 7343

NCBI Accession: NM_014233, NP_055048

UniProt: P17480