SSRP1 antibody (Middle Region)

Catalog No. ABIN486818

Product Details anti-SSRP1 Antibody

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSRP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SSSNEGDSDR DEKKRKQLKK AKMAKDRKSR KKPVEVKKGK DPNAPKRPMS

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against SSRP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SSRP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 709 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SSRP1

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Alternative Name: SSRP1 (SSRP1 Products)

Background: SSRP1 is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. SSRP1 contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. It also functions as a co-activator of the transcriptional activator p63. The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FACT, FACT80, T160
Protein Interaction Partner: SUMO2, SUMO3, ATRX, UBC, EED, RNF2, SEC24C, CUL1, CSDE1, ATP6V1B2, DIABLO, SBNO1, PBDC1, EXOSC10, CSNK2A1, PNKP, VCAM1, SRPK1, ITGA4, FMNL1, SUPT16H, CENPA, LEO1, PAF1, PHGDH, SF3B1, NCSTN, RTF1, RBM14, SAP18, CTR9, S100A9, NOP2, HMGB1, HIST1H2BB, DHX9, C
Protein Size: 709

Molecular Weight: 81 kDa

Gene ID: 6749

NCBI Accession: NM_003146, NP_003137

UniProt: Q08945

Pathways: Chromatin Binding