B-Cells antibody (FITC)
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- Target
- B-Cells
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- Detects a glycoprotein of 105kD found on circulating B-lymphocytes. Reacts with HRIK and Raji cell lines. Clinical Expression: B cell prolymphocytic leukemia (B-PLL): strongly positive, Hairy cell leukemia (HCL): strongly positive, Hairy cell leukemia variant (HCL-V): strongly positive, Splenic lymphoma with villous lymphocytes (SLVL): positive, B cell chronic lymphocytic leukemia (B-CLL): negative to weakly positive. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells.
- Purification
- Purified
- Immunogen
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The human B-lymphoblastoid cell line, HRIK.
Type of Immunogen: Cells - Isotype
- IgM
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- Application Notes
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Approved: Flo
Usage: Suitable for use in Flow Cytometry. Flow Cytometry: Neat. Use 10 μL per 100 μL of whole blood, or 1x1E6 peripheral blood mononuclear cells (PBMC) in 100 μL buffer. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied in 1.0 mL 0.1 M Tris-HCl, 0.5 M sodium chloride, 10 mM glycine, pH 8.0, 0.2 % BSA, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- B-Cells
- Alternative Name
- B Cell
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